ELISA KIT FOR CORTISOL

RAW MATERIAL

Antibody-coated tubes, Assay Buffer

Substrate buffer, Substrate

Enzyme Conjugate, Biochemicals

PLANT & MACHINERY

Elisa reader, Freezer, Ultracentrifuge

HPLC system, Colorimeter, Incubator

Polystyrene tubes, Microscope

Cortisol is a secretary product and plays an important role in maintaining the metabolic activity of the body. Estimation of cortisol is one of the most useful parameters to assess hormonal disorders such as Cushing’s syndrome, Addison’s disease, Adrenal tumours and congenital adrenal hyperplasia.

The enzyme linked immunosorbant assay (ELISA) that is developed is a quantitative in-vitro diagnostic method for measuring corticol inserum. It is a simple two-hour ELISA method for direct measurement of cortisol inserum. ELISA kit based on our technology is quick, specific and sensitive. The major components of this test have been found stable at specific temperature for certain period.

EVALUATION OF THE KIT

The performance of the kit was compared with imported kits obtained from (a) FENZIA, Onion Diagnostics, Finland and (b) Boehringer Mannheim Ltd-GmbH, Germany. These gave correlation coefficients of 0.81 and 0.78 respectively. These kits were evaluated at (a) Private Research Laboratory, Centre for Reproductive Biology and Molecular Endocrinology, IISc, Bangalore, (b) Department Of Endocrinology, Dr. A.L.M. Postgraduate Institute of Basic Medical Sciences, Tasamani, Madras, (c) Department Of Endocrinology, Postgraduate Institute of Medical Education & Research, Chandigarh and at (d) Tata Medical Research Centre, Jamshedpur.

A 100 tube Cortisol Kit is likely to cost approximately Rs.1500/-. Marketing costs will be extra.

NRDC

National Research Development Corporation, a Government of India Enterprise, is a premier Technology Transfer Corporation with four decades of experience. It has helped establish over one thousand projects in the small and medium scale sector. The supply of technologies and services to entrepreneurs extend both in the developing and developed countries like USA, Germany, Malaysia, Burma, Brazil, Nepal, Senegal, Madagascar, Indonesia, Philippines, Vietnam, Sri Lanka, Kenya, Bangladesh and Egypt.

Advantages of Indian technologies

Services offered by NRDC




NATIONAL RESEARCH DEVELOPMENT  CORPORATION
(A Government of India Enterprise)
20-22, Zamroodpur Community Centre,
Kailash Colony Extension, New Delhi-110 048. (INDIA)
Fax +91 11 29240409/29240410/29230506  Tel  +91 11 29240401 to 29240408

E Mail: write2@nrdcindia.com
Web Site :
www.nrdcindia.com